ITS2_Box1_PCR1_8

PCR 1 amplifying ITS2 marker for Box 1 - O.Fav deep

Protocol

Samples:

  • OFAV = Orbicella faveolata
  • OFRA = Orbicella franksi
  • S = Shallow
  • P = Deep
Species Treatment ID
OFAV P 9EV4
OFAV P 9EV40
OFAV P 9EV5
OFAV P 9GT35
OFAV P 9GT4
OFAV P 9GT46
OFAV P 9IR44
OFAV P 9JQ4
OFAV P 9JQ42
OFAV P 9JQ431
OFAV P 9JQ53
OFAV P AZ
OFAV P CX
OFAV P DW

Master Mix:

Component Volume for 1 rxn Volume for 5 rxn (+1% error) in triplicate
2x MyTaq Mix 12.5 uL 577.50 uL
Molecular Water 9.5 uL 438.90 uL
ITS2 Primer Forward (10 uM) 1.0 uL 46.2 uL
ITS2 Primer Reverse (10 uM) 1.0 uL 46.2 uL

Strip Tube Set Up:

Strip (replicate) Number A B C D E F G H
1 9EV4 9EV40 9EV5 9GT35 9GT4 9GT46 9IR44 9JQ4
2 9EV4 9EV40 9EV5 9GT35 9GT4 9GT46 9IR44 9JQ4
3 9EV4 9EV40 9EV5 9GT35 9GT4 9GT46 9IR44 9JQ4
4 9JQ42 9JQ431 9JQ53 AZ CX DW    
5 9JQ42 9JQ431 9JQ53 AZ CX DW    
6 9JQ42 9JQ431 9JQ53 AZ CX DW    

Gel Map:

See Box1_PCR7 for combined gel images

Written on June 2, 2022