ITS2_Box1_PCR1_3

PCR 1 amplifying ITS2 marker for Box 1 - O.Fav deep

Protocol

Samples:

  • OFAV = Orbicella faveolata
  • OFRA = Orbicella franksi
  • S = Shallow
  • P = Deep
Species Treatment ID
OFAV P 352
OFAV P 368
OFAV P 371
OFAV P OHS4
OFAV P 2HS2
OFAV P 2HS6
OFAV P 9GT4

Master Mix:

Component Volume for 1 rxn Volume for 4 rxn (+1% error) in triplicate
2x MyTaq Mix 12.5 uL 288.75 uL
Molecular Water 9.5 uL 219.45 uL
ITS2 Primer Forward (10 uM) 1.0 uL 23.10 uL
ITS2 Primer Reverse (10 uM) 1.0 uL 23.10 uL

Strip Tube Set Up:

Strip (replicate) Number A B C D E F G
1 352 368 371 OHS4 2HS2 2HS6 9GT4
2 352 368 371 OHS4 2HS2 2HS6 9GT4
3 352 368 371 OHS4 2HS2 2HS6 9GT4

Gel Map:

Failed: 352, 9GT4

Written on April 1, 2022